Author: Gardner, Shea N.; Hiddessen, Amy L.; Williams, Peter L.; Hara, Christine; Wagner, Mark C.; Colston, Bill W.
Title: Multiplex primer prediction software for divergent targets Document date: 2009_9_16
ID: 7658dmvk_15
Snippet: To predict how contamination with human DNA might affect the ability to detect specific amplification of viruses, the average number of amplicons for the human genome was also predicted based on these primer sets with 20 primers per bin ( Figure 4 ). The effect of reducing the multiplex size to 10 primers per reaction on human genome amplification was calculated by dividing priming sets in half, with 10 primers per reaction......
Document: To predict how contamination with human DNA might affect the ability to detect specific amplification of viruses, the average number of amplicons for the human genome was also predicted based on these primer sets with 20 primers per bin ( Figure 4 ). The effect of reducing the multiplex size to 10 primers per reaction on human genome amplification was calculated by dividing priming sets in half, with 10 primers per reaction.
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