Selected article for: "MEGA software and phylogenetic tree"

Author: Muhammad, Azharuddin; Toufeeq, Shahzad; Yu, Hai-Zhong; Wang, Jie; Zhang, Shang-Zhi; Li, Bing; Li, Zhen; Yang, Li-Ang; Hu, Pei; Ma, Yan; Xu, Jia-Ping
Title: Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection
  • Document date: 2019_2_2
  • ID: 2s3x6sj8_14
    Snippet: The iTRAQ-labeling quantitative proteomic technique was used to identify differentially expressed proteins in P50 (susceptible strain) and near-isogenic line BC9 (resistant strain) according to the mass spectra data (Yu et al. 2017b ). Finally, Bmp38 and BmS6K were identified based on National Center for Biotechnology Information nonredundant (NCBI-nr) database using basic local alignment search tool. Online ExPASy was used for the analysis of is.....
    Document: The iTRAQ-labeling quantitative proteomic technique was used to identify differentially expressed proteins in P50 (susceptible strain) and near-isogenic line BC9 (resistant strain) according to the mass spectra data (Yu et al. 2017b ). Finally, Bmp38 and BmS6K were identified based on National Center for Biotechnology Information nonredundant (NCBI-nr) database using basic local alignment search tool. Online ExPASy was used for the analysis of isoelectric point (IP) and molecular weight (MW) of Bmp38 and BmS6K (http:// web.expasy.org/compute_pi/). SMART was used for the conserved domain (http://smart.embl-heidelberg.de/). To build the phylogenetic tree MEGA 6.0 software was used, following the neighbor-joining method with 1,000-fold bootstrap (Tamura et al. 2013 ).

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