Author: Gardner, Shea N.; Hiddessen, Amy L.; Williams, Peter L.; Hara, Christine; Wagner, Mark C.; Colston, Bill W.
Title: Multiplex primer prediction software for divergent targets Document date: 2009_9_16
ID: 7658dmvk_46
Snippet: To confirm that the amplicon observed in gel analysis was a specifically amplified product from vaccinia virus, Lister strain, we sequenced the product according to the procedures described above. An analysis of the high-quality sequence read data taken from the electropherogram yielded a 95% identity (maximum) to vaccinia virus, Lister strain (AY678276.1) with a query The forward and reverse primers (FP, RP) in bold are predicted to amplify vacc.....
Document: To confirm that the amplicon observed in gel analysis was a specifically amplified product from vaccinia virus, Lister strain, we sequenced the product according to the procedures described above. An analysis of the high-quality sequence read data taken from the electropherogram yielded a 95% identity (maximum) to vaccinia virus, Lister strain (AY678276.1) with a query The forward and reverse primers (FP, RP) in bold are predicted to amplify vaccinia Lister with the indicated product length (617-bp amplicon size).
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