Genomics and Proteomics of Mycobacteriophage Patience, an Accidental Tourist in the Mycobacterium Neighborhood

Pope, Welkin H.; Jacobs-Sera, Deborah; Russell, Daniel A.; Rubin, Daniel H. F.; Kajee, Afsana; Msibi, Zama N. P.; Larsen, Michelle H.; Jacobs, William R.; Lawrence, Jeffrey G.; Hendrix, Roger W.; Hatfull, Graham F.

A similar scenario is seen within Patience gene 20, where two peptides corresponding to a 171-bp open reading frame on the same strand were identified with high confidence (see Fig. S1B in the supplemental material). Peptides corresponding to the predicted N terminus were not identified, although a start codon with a strong ribosome binding site is present (Fig. S2B) . The corresponding segment of Patience gp20 is conserved in Barnyard gp18 and Konstantine gp17, but the second open reading frame is not. Presumably, either expression of embedded out-of-frame genes is more common than anticipated or these reflect expression artifacts resulting from the use of noncognate host transcription and translation systems.