Murine Cytomegalovirus Is Regulated by a Discrete Subset of Natural Killer Cells Reactive with Monoclonal Antibody to Ly49h

Daniels, Keith A.; Devora, Gene; Lai, Wayne C.; O'Donnell, Carey L.; Bennett, Michael; Welsh, Raymond M.

Viruses. The Smith strain of MCMV was propagated in vivo in salivary glands of Balb/c mice (14) . Infected salivary gland homogenates were diluted in HBSS (Life Technologies) and, depending on the experiment, mice were inoculated intraperitoneally with 3 ϫ 10 3 to 2 ϫ 10 4 plaque-forming units (PFUs) of MCMV, as titrated on mouse embryonic fibroblast monolayers. The Armstrong strain of lymphocytic choriomeningitis virus (LCMV) and its more widely disseminating clone 13 variant were propagated in baby hamster kidney cells, and ‫ف‬ 5 ϫ 10 4 PFUs, as titrated on vero cell monolayers, were used for inoculations (23) . Vaccinia virus (VV), WR strain, was propagated in L929 cells, purified over sucrose gradients, and inoculated at doses of 10 6 PFUs, as titrated on vero cells (24) . Mouse hepatitis virus (MHV), strain A-59, was propagated in L929 cells and, unless otherwise stated, was inoculated into mice at doses of 7 ϫ 10 5 PFUs, as assayed on L929 cell monolayers (25) .