Selected article for: "GST tag and recombinant protein"
Author: Kim, Sae-Hae; Cho, Byeol-Hee; Lee, Kyung-Yeol; Jang, Yong-Suk
Title: N-terminal Domain of the Spike Protein of Porcine Epidemic Diarrhea Virus as a New Candidate Molecule for a Mucosal Vaccine
  • Document date: 2018_6_15
    • ID: 4vx7ez7s_19
    Snippet: The NTD 231-501 of the PEDV S1 protein has been shown to exhibit sugar-binding capability, as determined using a mucin-binding assay (8) . We also monitored the mucin-binding ability of our recombinant NTD 231-501 protein, because it was purified under denaturing conditions and contains a GST tag (Fig. 3A) . Recombinant NTD 231-501 exhibited significantly (p<0.05) higher mucin binding ability compared with the control (an unrelated protein: a rec.....
    Document: The NTD 231-501 of the PEDV S1 protein has been shown to exhibit sugar-binding capability, as determined using a mucin-binding assay (8) . We also monitored the mucin-binding ability of our recombinant NTD 231-501 protein, because it was purified under denaturing conditions and contains a GST tag (Fig. 3A) . Recombinant NTD 231-501 exhibited significantly (p<0.05) higher mucin binding ability compared with the control (an unrelated protein: a recombinant nonstructural 3 protein of dengue virus). Consequently, we speculated that recombinant NTD 231-501 of the PEDV S1 protein has mucin-binding ability and may induce PEDV S1 Ag-specific immune responses after immunization.

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