Selected article for: "blot analysis and horseradish peroxidase"
Author: Kim, Sae-Hae; Cho, Byeol-Hee; Lee, Kyung-Yeol; Jang, Yong-Suk
Title: N-terminal Domain of the Spike Protein of Porcine Epidemic Diarrhea Virus as a New Candidate Molecule for a Mucosal Vaccine
  • Document date: 2018_6_15
    • ID: 4vx7ez7s_8
    Snippet: For western blot analysis, the protein lysate was treated with or without isopropyl β-D-1-thiogalactopyranoside, subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene fluoride membranes. Immunoblotting was performed using an anti-GST Ab (Santa Cruz Biotechnology, Dallas, TX, USA). For visualization, a horseradish peroxidase (HRP)-conjugated secondary Ab was added, and bands were detected us.....
    Document: For western blot analysis, the protein lysate was treated with or without isopropyl β-D-1-thiogalactopyranoside, subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene fluoride membranes. Immunoblotting was performed using an anti-GST Ab (Santa Cruz Biotechnology, Dallas, TX, USA). For visualization, a horseradish peroxidase (HRP)-conjugated secondary Ab was added, and bands were detected using an enhanced chemiluminescence detection kit (GE Healthcare Life Sciences).

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