Selected article for: "16S rRNA gene and rRNA gene"
Author: Neumann, Roland P.; Hilty, Markus; Xu, Binbin; Usemann, Jakob; Korten, Insa; Mika, Moana; Müller, Loretta; Latzin, Philipp; Frey, Urs
Title: Nasal microbiota and symptom persistence in acute respiratory tract infections in infants
  • Document date: 2018_12_3
    • ID: 4rol2ogb_7
    Snippet: Nasal swabs were prospectively taken at the onset of the first ARI and 3 weeks later. Nasal swabs were further analysed by amplification of the variable regions V3 to V5 of the bacterial 16S ribosomal RNA (rRNA) gene by PCR and 454 Titanium FLX sequencing, as described previously [15] . We defined a cut-off value of 70 reads per sample. The reads from 454 Titanium amplicon sequencing were submitted to the European Nucleotide Archive (accession nu.....
    Document: Nasal swabs were prospectively taken at the onset of the first ARI and 3 weeks later. Nasal swabs were further analysed by amplification of the variable regions V3 to V5 of the bacterial 16S ribosomal RNA (rRNA) gene by PCR and 454 Titanium FLX sequencing, as described previously [15] . We defined a cut-off value of 70 reads per sample. The reads from 454 Titanium amplicon sequencing were submitted to the European Nucleotide Archive (accession number: PRJEB20454). Details of the microbiota analysis, the estimation of relative bacterial abundances and the microbiota clustering can be found the supplementary methods.

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