Author: Xiao, Rui; Chen, Rongchang
Title: Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury Document date: 2017_4_7
ID: 3e72pzui_5
Snippet: Animals and experimental protocol. Male C57BL/6 mice, (20±2 g, 6-8 weeks and free of murine specific pathogens), were obtained from the Experimental Animal Center of Guangdong Province (Guangzhou, China). The mice were housed in a specific pathogen free animal house at a temperature of 22±2˚C Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury and a relative humidity of 55±5% with a .....
Document: Animals and experimental protocol. Male C57BL/6 mice, (20±2 g, 6-8 weeks and free of murine specific pathogens), were obtained from the Experimental Animal Center of Guangdong Province (Guangzhou, China). The mice were housed in a specific pathogen free animal house at a temperature of 22±2˚C Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury and a relative humidity of 55±5% with a 12:12 h light/dark cycle. Throughout the experimental process, the mice were housed in a laminar flow cabinet and maintained on standard laboratory food ad libitum. The study was approved by the ethics committee of Guangzhou University of Traditional Chinese Medicine. Experiments were conducted on 42 wild-type mice, which were divided into 7 groups. The protocol for the control group was spontaneous breathing for 2 h. The protocol for the high peak inflation pressure (high-PIP) group was breathing under the condition of 50 cm H 2 O of PIP and 2. Table I .
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