Author: Lambert, Stephen Bernard; Ware, Robert S; Cook, Anne L; Maguire, Frances A; Whiley, David M; Bialasiewicz, Seweryn; Mackay, Ian M; Wang, David; Sloots, Theo P; Nissen, Michael D; Grimwood, Keith
Title: Observational Research in Childhood Infectious Diseases (ORChID): a dynamic birth cohort study Document date: 2012_10_31
ID: 06e9lkwl_21
Snippet: Nose and nappy specimens are batch tested for viruses using previously validated and reported realtime polymerase chain reaction (PCR) assays, or PCR assays developed specifically for this study (Table) . Reverse transcription precedes PCR for RNA viruses. Specimens are spiked with a known concentration of Equine Herpes Virus (EHV) before extraction to assess extraction efficiency and for the presence of PCR inhibitors. The quality of respiratory.....
Document: Nose and nappy specimens are batch tested for viruses using previously validated and reported realtime polymerase chain reaction (PCR) assays, or PCR assays developed specifically for this study (Table) . Reverse transcription precedes PCR for RNA viruses. Specimens are spiked with a known concentration of Equine Herpes Virus (EHV) before extraction to assess extraction efficiency and for the presence of PCR inhibitors. The quality of respiratory and stool specimen collection are assessed by evaluating for the presence of a marker of human genomic DNA, ERV3. 37 Appropriate positive and negative controls are included in every run. Nasal and nappy samples from children during illness periods where a known agent cannot be identified will be used for further investigations for the presence of as yet unidentified organisms.
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