Selected article for: "amino acid and single amino acid"
Title: Oligomerization of a membrane protein correlates with its retention in the Golgi complex
  • Document date: 1993_9_2
    • ID: 5z1xminb_12
    Snippet: Gml and related mutants were generated using the Kunkel method of oligonucleotide-direeted mutagenesis as described previously (Swift and Machamer, 1991; Machamer et al., 1993) . Point mutants were named by appending the original amino acid (in single letter code) followed by the new amino acid. ,m (Guan et al., 1988a) and TMR (Doms et al., 1988) were obtained from Jack Rose (Yale University). Construction of ,~mlG was described previously (Swift.....
    Document: Gml and related mutants were generated using the Kunkel method of oligonucleotide-direeted mutagenesis as described previously (Swift and Machamer, 1991; Machamer et al., 1993) . Point mutants were named by appending the original amino acid (in single letter code) followed by the new amino acid. ,m (Guan et al., 1988a) and TMR (Doms et al., 1988) were obtained from Jack Rose (Yale University). Construction of ,~mlG was described previously (Swift and Machamer, 1991) . tGml was constructed from Gml by inserting a stop codon at position 487 using site-directed mutagenesis (Kunkel et al., 1987) .

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