Selected article for: "er exit and indirect immunofluorescence"
Title: Oligomerization of a membrane protein correlates with its retention in the Golgi complex
  • Document date: 1993_9_2
    • ID: 5z1xminb_40
    Snippet: indirect immunofluorescence in transiently transfected COS-7 cells, some Golgi staining was observed, although a significant amount of the protein was found in the ER. Unlike Grid, tGml did not form an SDS-resistant oligomer when expressed in COS-'/or HeLa cells, and did not sediment as a large oligomer in sucrose gradients (although it did apparently form trimers, not shown). However, because tGml appears to exit the ER very slowly (like TMR), t.....
    Document: indirect immunofluorescence in transiently transfected COS-7 cells, some Golgi staining was observed, although a significant amount of the protein was found in the ER. Unlike Grid, tGml did not form an SDS-resistant oligomer when expressed in COS-'/or HeLa cells, and did not sediment as a large oligomer in sucrose gradients (although it did apparently form trimers, not shown). However, because tGml appears to exit the ER very slowly (like TMR), the kinetics of oligomer formation may have been too slow to detect.

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