Origin, Evolution, and Virulence of Porcine Deltacoronaviruses in the United States

Ma, Yuanmei; Zhang, Yu; Liang, Xueya; Lou, Fangfei; Oglesbee, Michael; Krakowka, Steven; Li, Jianrong

Animal experiment 1: Gn piglets challenged with Ohio CVM intestinal contents. Five 19-day-old Gn piglets were randomly divided into two groups. Two piglets in group 1 served as uninfected controls. The three piglets in group 2 were infected with PdCV. Prior to oral challenge, preinoculation fecal and oronasal swabs and blood for serum were collected for virus detection from the three piglets of group 2. Piglets were pretreated with parenteral ranitidine (25 mg/kg of body weight). The three piglets in group 2 were orally challenged with 5.0 ml of filter-sterilized intestinal contents from the PdCV-positive piglet described above, representing 10 6 genomic RNA copies of PdCV Ohio CVM1. The two piglets in group 1 were challenged with 5 ml of filtersterilized intestinal contents from normal piglets, negative for known porcine viruses.