Author: Ma, Yuanmei; Zhang, Yu; Liang, Xueya; Lou, Fangfei; Oglesbee, Michael; Krakowka, Steven; Li, Jianrong
Title: Origin, Evolution, and Virulence of Porcine Deltacoronaviruses in the United States Document date: 2015_3_10
ID: 6o7j5k9n_37
Snippet: Animal experiment 2: Gn piglets challenged with the PdCV MI strain. Eight 10-day-old Gn piglets were randomly divided into two isolators. There were a total of 5 Gn piglets (C1 to C5) in isolator 1, and each piglet was housed in an enclosure that prevented direct animal-to-animal contact (Fig. 7A ). Three piglets (C1, C3, and C5) in isolator 1 were orally challenged with 5 ml of DMEM containing 10 6 PFU of the PdCV MI strain. The remaining two pi.....
Document: Animal experiment 2: Gn piglets challenged with the PdCV MI strain. Eight 10-day-old Gn piglets were randomly divided into two isolators. There were a total of 5 Gn piglets (C1 to C5) in isolator 1, and each piglet was housed in an enclosure that prevented direct animal-to-animal contact (Fig. 7A ). Three piglets (C1, C3, and C5) in isolator 1 were orally challenged with 5 ml of DMEM containing 10 6 PFU of the PdCV MI strain. The remaining two piglets (C2 and C4) in isolator 1 did not receive any treatment. This experiment was designed to determine whether PdCV can be transmitted from the virus-inoculated piglets to the uninoculated piglets. In isolator 2, three piglets were orally inoculated with 5 ml of DMEM and served as uninfected controls.
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