Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

Li, Xinyu; Huang, Lei; Gao, Weijie

Epithelial-mesenchymal transition (EMT), a process reported to be important in cancer metastasis, enhances cancer motility and dissemination through the disruption of intercellular junctions [10] . Thus, to further investigate the molecular mechanism by which TRIM55 regulates migration of HCC cells, we used immunofluorescence and Western blot assay to detect expression change of E-cadherin and Vimentin under the condition of TRIM55 overexpression. As expected, we found the expression of E-cadherin was increased and expression of Vimentin was decreased in HCC cells when TRIM55 was overexpressed. Similarly, as MMP2 was reported to be important for cell invasion through regulating cell matrix degradation [11] , and then we found that the expression of MMP2 was significantly decreased after TRIM55 was overexpressed in HCC cells. Therefore, our study found that TRIM55 can regulate migration and invasion of HCC cells through EMT and MMP2 pathway.