Selected article for: "agarose gel electrophoresis and recombinant plasmid"
Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
  • Document date: 2018_3_23
    • ID: 4nphwznx_23
    Snippet: Gene sequencing analysis and restriction endonuclease digestion were used to validate the construction of the recombinant plasmid pcDNA3.1-M-rvfv. The pcDNA3.1-M-rvfv plasmid was digested with the Not I and Kpn I enzymes and evaluated by performing agarose gel electrophoresis (Fig. 2) . The results showed two nucleic acid bands, at approximately 3200 bp and 5500 bp, which were consistent with the target gene fragment (3218 bp) and the pcDNA3.1 em.....
    Document: Gene sequencing analysis and restriction endonuclease digestion were used to validate the construction of the recombinant plasmid pcDNA3.1-M-rvfv. The pcDNA3.1-M-rvfv plasmid was digested with the Not I and Kpn I enzymes and evaluated by performing agarose gel electrophoresis (Fig. 2) . The results showed two nucleic acid bands, at approximately 3200 bp and 5500 bp, which were consistent with the target gene fragment (3218 bp) and the pcDNA3.1 empty plasmid (5428 bp), respectively. This result verified the correct insertion of the target fragment. The gene fragment obtained in this experiment and the target gene sequence obtained by using gene sequencing were 100% homologous, indicating that the pcDNA3.1-M-rvfv recombinant plasmid was correctly constructed.

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