Selected article for: "acid instillation and mechanical ventilation"
Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation
  • Document date: 2020_3_25
    • ID: ebwxryai_15
    Snippet: Mice were studied in three different conditions: baseline, 4 hours after chlorhydric acid instillation without mechanical ventilation and 4 hours after acid instillation including 2 hours of mechanical ventilation. Lungs were removed after exsanguination of anesthetized animals. A laparotomy was performed, the renal artery sectioned, the thorax opened and the heart-lungs removed in bloc. The left lung was instilled with 250 microliters of 4% phos.....
    Document: Mice were studied in three different conditions: baseline, 4 hours after chlorhydric acid instillation without mechanical ventilation and 4 hours after acid instillation including 2 hours of mechanical ventilation. Lungs were removed after exsanguination of anesthetized animals. A laparotomy was performed, the renal artery sectioned, the thorax opened and the heart-lungs removed in bloc. The left lung was instilled with 250 microliters of 4% phosphate-buffered paraformaldehyde, immersed in the same fixative for 24 hours, and then stored in 50% ethanol. The right lung was immediately frozen at -80°C for biochemical analyses.

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