Cellular senescence limits acute lung injury induced by mechanical ventilation

Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta

To explore the mechanisms involved in the later development of the senescent response, a clinically-relevant experimental model, acid aspiration-and mechanical ventilation induced lung injury, was tested. Chlorhydric acid instillation and mechanical ventilation induced a significant increase in lung damage and inflammation, assessed by histological scores (Figure 2A To confirm the incidence of SAHF in patients, lung tissue from autopsies of criticallyill patients with and without lung injury and mechanical ventilation (Supplementary Table 2 ) were stained with antibodies against Macro-H2A. There were no differences in age between the three groups of patients (62±6, 61±11 and 54±10 years for patients without ARDS or mechanical ventilation, with ARDS but without . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005983 doi: bioRxiv preprint mechanical ventilation and ARDS and ventilation respectively, p=0.17 in ANOVA).