Selected article for: "accession number and specific pcr"
Author: Timurkan, Mehmet Ozkan; Aydin, Hakan; Sait, Ahmet
Title: Identification and Molecular Characterisation of Bovine Parainfluenza Virus-3 and Bovine Respiratory Syncytial Virus - First Report from Turkey
  • Document date: 2019_6_12
    • ID: 2sm0pgt7_14
    Snippet: Sequencing. After PCR, the samples with the clearest virus-specific PCR bands were subjected to sequencing. Samples were sequenced (with the Sanger sequencing method, bi-directionally with forward and reverse primers) by the Pendik Veterinary Control Institute, Istanbul, Turkey. Obtained sequences were submitted to GenBank as bovine respirovirus 3 isolate BPIV3/TR/Erz/2014 under accession number KY511410 and bovine orthopneumovirus isolate BRSV/T.....
    Document: Sequencing. After PCR, the samples with the clearest virus-specific PCR bands were subjected to sequencing. Samples were sequenced (with the Sanger sequencing method, bi-directionally with forward and reverse primers) by the Pendik Veterinary Control Institute, Istanbul, Turkey. Obtained sequences were submitted to GenBank as bovine respirovirus 3 isolate BPIV3/TR/Erz/2014 under accession number KY511410 and bovine orthopneumovirus isolate BRSV/TR/Erz/2014 under accession number KY499619, and then were analysed phylogenetically.

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