Author: Liu, Hong Yan; Gao, Xiaohu
Title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras Document date: 2013_11_7
ID: 0atfsivf_28
Snippet: Cytotoxicity assay. LNCaP cells were seeded in 96-well plate at 4 3 10 3 /well for 24 hours, and then treated with different concentrations of dsRBD-His 18 protein tag for 72 hours. CellTiter-Blue reagent (20 ml) was added into each well. After 4 h incubation at 37uC, cell viability was assessed by fluorescence intensity at 590 nm (excitation 570 nm) on a TECAN infinite M200 microplate reader......
Document: Cytotoxicity assay. LNCaP cells were seeded in 96-well plate at 4 3 10 3 /well for 24 hours, and then treated with different concentrations of dsRBD-His 18 protein tag for 72 hours. CellTiter-Blue reagent (20 ml) was added into each well. After 4 h incubation at 37uC, cell viability was assessed by fluorescence intensity at 590 nm (excitation 570 nm) on a TECAN infinite M200 microplate reader.
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