Author: SAKAI, Kosei; YONEZAWA, Tomohiro; YAMAWAKI, Hideyuki; OYAMADA, Toshifumi
Title: Alteration of somatostatin receptor 2 expression in canine mammary gland tumor Document date: 2015_5_18
ID: 765ufb5i_10
Snippet: We had conflicting results between immunohistochemical reactivity and protein level of SSTR2 in malignant tumors. In this study, the same primary antibody to SSTR2 was used for both immunohistochemistry and Western blotting. However, very little reactivity was observed in immunohistochemistry, while Western blotting detected a large amount of protein. Indeed, it is not so rare that there is some discrepancy between the results of immunohistochemi.....
Document: We had conflicting results between immunohistochemical reactivity and protein level of SSTR2 in malignant tumors. In this study, the same primary antibody to SSTR2 was used for both immunohistochemistry and Western blotting. However, very little reactivity was observed in immunohistochemistry, while Western blotting detected a large amount of protein. Indeed, it is not so rare that there is some discrepancy between the results of immunohistochemistry and Western blotting [6, 9] . Although further studies are needed to understand the reasons for these results, there are several hypotheses that could account for them. 1) In immunohistochemistry, the tissue is fixed in 10% neutral buffered formalin, and the epitope may be obscured or cross-linked due to experimental conditions. 2) Because Western blotting was performed after the reduction treatment, a conformational change in the protein may alter the intensity of immunoreactivity.
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