Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation Document date: 2020_3_25
ID: ebwxryai_26
Snippet: Cultured A549 cells were harvested, rinsed in PBS and fixated in ice-cold 70% ethanol for 30 minutes. Cells were later pelleted at 1300 rpms for 5 minutes at 4°C and washed twice with PBS. Cells were stained in a solution containing 0.02 mg/ml propidium iodide, 0.1% (v/v) Triton X-100 and 0.2mg/ml DNase-free RNase A in PBS, and incubated for at least 30 minutes at room temperature and protected from the light before analysis. 10,000 events were .....
Document: Cultured A549 cells were harvested, rinsed in PBS and fixated in ice-cold 70% ethanol for 30 minutes. Cells were later pelleted at 1300 rpms for 5 minutes at 4°C and washed twice with PBS. Cells were stained in a solution containing 0.02 mg/ml propidium iodide, 0.1% (v/v) Triton X-100 and 0.2mg/ml DNase-free RNase A in PBS, and incubated for at least 30 minutes at room temperature and protected from the light before analysis. 10,000 events were analyzed per sample (in triplicates).
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