Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_23
Snippet: Expression of the three different families of Alu elements has so far been poorly investigated. Based on cDNA analysis (15, 16) , the AluY elements are most abundantly expressed. We therefore chose to use in vitro transcribed AluY A and scAluY A RNAs derived from the Alu element in the human â£-fetoprotein gene (Table 1) together with human SRP9/14 (h9/14) to assemble AluY A and scAluY A RNPs, respectively. The RNPs were purified by gel filtrati.....
Document: Expression of the three different families of Alu elements has so far been poorly investigated. Based on cDNA analysis (15, 16) , the AluY elements are most abundantly expressed. We therefore chose to use in vitro transcribed AluY A and scAluY A RNAs derived from the Alu element in the human â£-fetoprotein gene (Table 1) together with human SRP9/14 (h9/14) to assemble AluY A and scAluY A RNPs, respectively. The RNPs were purified by gel filtration chromatography, and the integrity of the RNA moiety was confirmed by denaturing polyacrylamide gel electrophoresis followed by ethidium bromide staining (Supplementary Figure S1B ). Western blot analysis demonstrated that the AluY A and the scAluY A RNPs contained, respectively, two and one molecules of h9/14 per molecule of RNA (Supplementary Figure S1C ).
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