Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_49
Snippet: In parallel experiments in our laboratory, we have recently discovered that SRP9/14 can bind directly to 40S subunits and that binding to 40S is required for stress granule localization. Furthermore, we found that binding of SRP9/14 to 40S subunits and Alu RNA is mutually exclusive (24) . However, translation initiation is only inhibited by the Alu RNP and not by the protein alone, and both the RNA and protein moieties are essential for the inhib.....
Document: In parallel experiments in our laboratory, we have recently discovered that SRP9/14 can bind directly to 40S subunits and that binding to 40S is required for stress granule localization. Furthermore, we found that binding of SRP9/14 to 40S subunits and Alu RNA is mutually exclusive (24) . However, translation initiation is only inhibited by the Alu RNP and not by the protein alone, and both the RNA and protein moieties are essential for the inhibitory activity of the Alu RNP. To study the roles of the RNA and the protein components of the Alu RNP in inhibiting initiation, we performed binding experiments. High salt purified 40S and 60S subunits were incubated with the scAluY A RNP and with h9/14 alone, and the complexes fractionated on sucrose gradients.
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