Selected article for: "room temperature and substrate solution"

Author: Yen, Wei-Chen; Wu, Yi-Hsuan; Wu, Chih-Ching; Lin, Hsin-Ru; Stern, Arnold; Chen, Shih-Hsiang; Shu, Jwu-Ching; Tsun-Yee Chiu, Daniel
Title: Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling
  • Document date: 2019_11_2
  • ID: 6fw4thkq_19
    Snippet: The G6PD activity of whole blood was measured using a G6PD quantitative kit (Trinity Biotech) according to the manufacturer's instructions. In brief, 500 μl assay reagent and 5 μl whole blood were added to the cuvette and incubated for 5 min at room temperature. Then, 1 ml of the substrate solution was added to the cuvette and incubated for 2 min at 37°C. The reduction of NADP + was measured spectrophotometrically at 340 nm. The activity of G6.....
    Document: The G6PD activity of whole blood was measured using a G6PD quantitative kit (Trinity Biotech) according to the manufacturer's instructions. In brief, 500 μl assay reagent and 5 μl whole blood were added to the cuvette and incubated for 5 min at room temperature. Then, 1 ml of the substrate solution was added to the cuvette and incubated for 2 min at 37°C. The reduction of NADP + was measured spectrophotometrically at 340 nm. The activity of G6PD is expressed as U/10 12 erythrocytes (RBCs).

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