Selected article for: "cord spinal brain and spinal brain"
Title: In vivo analysis of glial cell phenotypes during a viral demyelinating disease in mice
  • Document date: 1989_11_1
    • ID: 4t98bah8_20
    Snippet: Lipids were extracted from adult C57 BI/6 whole brain and whole spinal cord as previously described (Magnani et al., 1982; Rougeon et al., 1986) . Briefly, 500 mg (wet weight) of tissue was homogenized with 1.5 ml H20, the homogenate was mixed with 5 ml methanol and 2.5 ml chloroform and stirred at room temperature for 30--45 min. The mixture was centrifuged for 15 min at 1,500 g. For the spinal cord, pellets were resuspended and recentrifuged a .....
    Document: Lipids were extracted from adult C57 BI/6 whole brain and whole spinal cord as previously described (Magnani et al., 1982; Rougeon et al., 1986) . Briefly, 500 mg (wet weight) of tissue was homogenized with 1.5 ml H20, the homogenate was mixed with 5 ml methanol and 2.5 ml chloroform and stirred at room temperature for 30--45 min. The mixture was centrifuged for 15 min at 1,500 g. For the spinal cord, pellets were resuspended and recentrifuged a second time. The supernatants were collected, evaporated to dryness, and suspended in chlomform/mcthanol (l:l).

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