Selected article for: "gel electrophoresis and horseradish peroxidase"
Author: Tornai, David; Furi, Istvan; Shen, Zu T.; Sigalov, Alexander B.; Coban, Sahin; Szabo, Gyongyi
Title: Inhibition of Triggering Receptor Expressed on Myeloid Cells 1 Ameliorates Inflammation and Macrophage and Neutrophil Activation in Alcoholic Liver Disease in Mice
  • Document date: 2018_10_29
    • ID: 35jfg45k_17
    Snippet: Whole-liver proteins were boiled in Laemmli's buffer. Samples were resolved in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel under reducing conditions, using a Tris-glycine buffer system; resolved proteins were transferred onto a nitrocellulose membrane. SYK proteins were detected by specific primary antibodies (SYK, 2712 [Cell Signaling]; phospho-SYK Y525/526 , ab58575 [Abcam]) followed by an appropriate secondary horseradish.....
    Document: Whole-liver proteins were boiled in Laemmli's buffer. Samples were resolved in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel under reducing conditions, using a Tris-glycine buffer system; resolved proteins were transferred onto a nitrocellulose membrane. SYK proteins were detected by specific primary antibodies (SYK, 2712 [Cell Signaling]; phospho-SYK Y525/526 , ab58575 [Abcam]) followed by an appropriate secondary horseradish peroxidase-conjugated immunoglobulin G antibody from Santa Cruz Biotechnology. β-actin, detected by an ab49900 antibody (Abcam), was used as a loading control. The specific immunoreactive bands of interest were visualized by chemiluminescence (Bio-Rad Laboratories) using the Fujifilm LAS-4000 luminescent image analyzer.

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