Author: Swatek, Kirby N.; Aumayr, Martina; Pruneda, Jonathan N.; Visser, Linda J.; Berryman, Stephen; Kueck, Anja F.; Geurink, Paul P.; Ovaa, Huib; van Kuppeveld, Frank J. M.; Tuthill, Tobias J.; Skern, Tim; Komander, David
Title: Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies Document date: 2018_3_6
ID: 3s86w4iw_20
Snippet: The here identified virus-induced GlyGly remnants on substrate proteins may lead to improvements in the detection of foot-andmouth disease (FMD). Vaccination is critical to the control FMD outbreaks; however, it is difficult to distinguish vaccinated from infected animals. Current strategies rely on ELISA-based methods to detect antibodies against nonstructural virus proteins in serum. Our findings suggest that GlyGly-modified proteins could also.....
Document: The here identified virus-induced GlyGly remnants on substrate proteins may lead to improvements in the detection of foot-andmouth disease (FMD). Vaccination is critical to the control FMD outbreaks; however, it is difficult to distinguish vaccinated from infected animals. Current strategies rely on ELISA-based methods to detect antibodies against nonstructural virus proteins in serum. Our findings suggest that GlyGly-modified proteins could also be used in ELISAs to detect antibodies against this epitope. Detection of antibodies against GlyGly modifications signifies enzymatic activity of Lb pro that would only be observed after viral infection and hence, distinguish infected from vaccinated animals. This utility of detecting FMDV infection may ease the economic burden imposed by FMD, particularly in developing countries, by providing a previously unrecognized biomarker for its detection.
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