Selected article for: "Macherey Nagel NucleoSpin RNA kit and NucleoSpin RNA kit"
Author: Strating, Jeroen R.P.M.; van der Linden, Lonneke; Albulescu, Lucian; Bigay, Joëlle; Arita, Minetaro; Delang, Leen; Leyssen, Pieter; van der Schaar, Hilde M.; Lanke, Kjerstin H.W.; Thibaut, Hendrik Jan; Ulferts, Rachel; Drin, Guillaume; Schlinck, Nina; Wubbolts, Richard W.; Sever, Navdar; Head, Sarah A.; Liu, Jun O.; Beachy, Philip A.; De Matteis, Maria A.; Shair, Matthew D.; Olkkonen, Vesa M.; Neyts, Johan; van Kuppeveld, Frank J.M.
Title: ITRACONAZOLE INHIBITS ENTEROVIRUS REPLICATION BY TARGETING THE OXYSTEROL-BINDING PROTEIN
  • Document date: 2015_1_29
    • ID: 3kmqy07w_15
    Snippet: [dT]-3', and 5'-GCACCUCAACUGUUCACAA[dT][dT]-3') were from Sigma. After 48 h, cells were infected with virus described under 'Virus infection', an MTS-assay (essentially as described under 'Multicycle CPE-reduction assay') was performed to evaluate effects of knockdown on cell viability, or RNA was isolated using the Nucleospin RNA kit (Macherey Nagel) and used to evaluate knockdown efficiency by qPCR analysis using the LightCycler 480 SYBR green .....
    Document: [dT]-3', and 5'-GCACCUCAACUGUUCACAA[dT][dT]-3') were from Sigma. After 48 h, cells were infected with virus described under 'Virus infection', an MTS-assay (essentially as described under 'Multicycle CPE-reduction assay') was performed to evaluate effects of knockdown on cell viability, or RNA was isolated using the Nucleospin RNA kit (Macherey Nagel) and used to evaluate knockdown efficiency by qPCR analysis using the LightCycler 480 SYBR green I Master kit (Roche) (primers used: OSBP 5'-ATCAAACAGGTCAACGAACGAG-3' and 5'-GGGTCTGGGCTAACATGAGGA-3', ORP4 5'-GAACCTGTGTCCGAGACGAC-3' and 5'-CCTGAGCTTGACTCTGACCC-3').

    Search related documents:
    Co phrase search for related documents
    • cell viability and CPE reduction assay: 1
    • cell viability and knockdown effect: 1, 2, 3, 4
    • cell viability and Multicycle CPE reduction assay: 1
    • cell viability and RNA kit: 1, 2
    • cell viability and virus infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • CPE reduction and Multicycle CPE reduction assay: 1
    • CPE reduction and virus infection: 1, 2
    • CPE reduction assay and Multicycle CPE reduction assay: 1
    • CPE reduction assay and virus infection: 1
    • knockdown effect and virus infection: 1, 2, 3, 4, 5
    • knockdown efficiency and virus infection: 1
    • Macherey Nagel Nucleospin RNA kit and Master kit: 1
    • Macherey Nagel Nucleospin RNA kit and Nucleospin RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • Macherey Nagel Nucleospin RNA kit and RNA kit: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • Macherey Nagel Nucleospin RNA kit and Roche Master kit: 1
    • Master kit and Nucleospin RNA kit: 1
    • Master kit and RNA kit: 1, 2, 3, 4
    • Master kit and Roche Master kit: 1, 2, 3
    • Nucleospin RNA kit and Roche Master kit: 1