Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_26
Snippet: Enrofloxacin separated by MNP-mAb conjugates was detected via HPLC using an XTerra RP18 column (Waters, Ireland). The chromatographic column was then equilibrated with a mixture of water-acetonitrile-methanol (800 : 170 : 30; v/v/v). The flow rate of the mobile phase was 1.2 mL/min. Dissociated samples (20 ïL) were injected into the HPLC system (Waters) and the eluate was monitored for ENR using a fluorescence detector (Waters) at an excitation.....
Document: Enrofloxacin separated by MNP-mAb conjugates was detected via HPLC using an XTerra RP18 column (Waters, Ireland). The chromatographic column was then equilibrated with a mixture of water-acetonitrile-methanol (800 : 170 : 30; v/v/v). The flow rate of the mobile phase was 1.2 mL/min. Dissociated samples (20 ïL) were injected into the HPLC system (Waters) and the eluate was monitored for ENR using a fluorescence detector (Waters) at an excitation of 278 nm and an emission of 455 nm.
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