Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_4
Snippet: Bovine serum albumin (BSA), enrofloxacin, ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, norfloxaicin, keyhole limpet hemocyanin (KLH), N-hydroxysuccinimide (NHS), triethylamine, carbonate-bicarbonate buffer, Tween 20, glutaraldehyde solution (Grade II, 25%), glycine, Freund's complete adjuvant/incomplete adjuvant, and 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC) were purchased from Sigma-Aldrich (USA). Goat anti-mouse Ig.....
Document: Bovine serum albumin (BSA), enrofloxacin, ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, norfloxaicin, keyhole limpet hemocyanin (KLH), N-hydroxysuccinimide (NHS), triethylamine, carbonate-bicarbonate buffer, Tween 20, glutaraldehyde solution (Grade II, 25%), glycine, Freund's complete adjuvant/incomplete adjuvant, and 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC) were purchased from Sigma-Aldrich (USA). Goat anti-mouse IgG was purchased from Abcam (UK). An EZ-Link Plus Activated Peroxidase kit was purchased from Thermo Scientific (USA). 3, 3', 5, 5'-tetra-methylbenzidine (TMB) solution was purchased from KPL (USA). Amine-functionalized MNPs (160 nm) were acquired from Nanobric (Korea). A HiTrap Protein G HP kit was obtained from GE Healthcare (UK).
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