Title: Membrane insertion of gap junction connexins: polytopic channel forming membrane proteins Document date: 1994_10_2
ID: 1gqffey0_9
Snippet: Construction of eucaryotic connexin expression vectors will be described in more detail in separate reports. Briefly, human/3t GJ protein was expressed in yeast cells (Saccharomyces cerevisiae) after the human/~1 GJ eDNA was cloned into the BamHI site of the yeast expression vector YEP G2 containing a polyadenylation signal, a 2-/~ plasmid origin of replication, and a galactose regulatable promoter......
Document: Construction of eucaryotic connexin expression vectors will be described in more detail in separate reports. Briefly, human/3t GJ protein was expressed in yeast cells (Saccharomyces cerevisiae) after the human/~1 GJ eDNA was cloned into the BamHI site of the yeast expression vector YEP G2 containing a polyadenylation signal, a 2-/~ plasmid origin of replication, and a galactose regulatable promoter.
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