miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao

NATURE COMMUNICATIONS | DOI: 10.1038/ncomms3903 ARTICLE with low sensitivity and poor resolution, which lack vigor in revealing the abundance of each miRNA in the background of the entire miRNome. At present, MPSS of miRNAs can identify the entire miRNome in-depth and explore miRNA expression differences as well as the individual miRNA abundance in a given tissue or cell type under a certain physiological or pathological context. We have carried out Illumina sequencing analysis to define the miRNomes in human liver and hepatocellular carcinoma 36 and also in human NK cells 10 analysis of miRNome has viewed of expressed miRNAs over a wide dynamic range of expression levels in several kinds of tissues and cell types such as human embryonic stem cells, human B cells and mouse NK cells 20, 38, 39 . However, the full scale of miRNomes in various DC subsets and HSCs remains to be fully identified. In the present study, the miRNomes of DC development and differentiation in vitro and in vivo, including HSCs, imDCs, maDCs and DCreg have been revealed. Our results reveal that, during DC development and differentiation, miRNA expression spectrums were similar, but miRNA expression profiles changed significantly, especially in DC development and differentiation processes. The miRNAs, preferentially expressed at one or two stages, may raise interesting future work to elucidate the detailed roles of these miRNAs in the regulation of DC development and differentiation. Epigenetic modification involved in the regulation of gene expression has been widely investigated, mainly focusing on protein-coding genes. Many of these genes with tissue-specific expression undergo epigenetic reprogramming during cell development and differentiation 40, 41 . However, epigenetic regulation of non-protein-coding genes, especially miRNAs, is not yet clear.