Author: Cordey, Samuel; Thomas, Yves; Suter, Patricia; Kaiser, Laurent
Title: Pilot Evaluation of RT-PCR/Electrospray Ionization Mass Spectrometry (PLEX-ID/Flu assay) on Influenza-Positive Specimens Document date: 2012_5_9
ID: 085v7n6k_1
Snippet: The World Health Organization (WHO) Global Influenza Surveillance and Response System provides essential information on the types and variants of influenza viruses circulating worldwide. Direct detection and identification of influenza strains from clinical samples are mostly performed by using specific real-time PCR assays and, less frequently, by culture-based methods [1, 2] . Genotyping or phenotyping characterization of positive cases is perf.....
Document: The World Health Organization (WHO) Global Influenza Surveillance and Response System provides essential information on the types and variants of influenza viruses circulating worldwide. Direct detection and identification of influenza strains from clinical samples are mostly performed by using specific real-time PCR assays and, less frequently, by culture-based methods [1, 2] . Genotyping or phenotyping characterization of positive cases is performed by sequencing classical PCR amplicons (requiring multiple primer pairs), followed by phylogenetic analysis, or hemagglutinin inhibition assay, respectively.
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