Author: Larouche-Lebel, Éva; Loughran, Kerry A.; Oyama, Mark A.; Solter, Phil F.; Laughlin, Danielle S.; Sánchez, Melissa D.; Assenmacher, Charles-Antoine; Fox, Philip R.; Fries, Ryan C.
Title: Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease Document date: 2019_6_28
ID: 6ek80l79_6
Snippet: The imprecision of the measurement of MCA was determined at the five standard solution concentrations used in the assay. The concentrated MCA standard solution was diluted in assay linearity buffer. To assess within run performance, 100 µL of each MCA concentration was added to duplicate ELISA plate wells and fluorescence (Ex/Em = 320/420 nm) measured every 8 minutes for 120 minutes. The experiment was repeated five times on five different days .....
Document: The imprecision of the measurement of MCA was determined at the five standard solution concentrations used in the assay. The concentrated MCA standard solution was diluted in assay linearity buffer. To assess within run performance, 100 µL of each MCA concentration was added to duplicate ELISA plate wells and fluorescence (Ex/Em = 320/420 nm) measured every 8 minutes for 120 minutes. The experiment was repeated five times on five different days to determine the between run test performance. The coefficient of variation of the relative fluorescence units (RFUs) of each concentration was then calculated (Supplemental Table 1 ).
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