2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

The study was a single-dose, multi-center, clinical trial. Foals aged 0-96 hours with sepsis as defined by a sepsis score ≥ 11 or sick (nonseptic) defined as rectal temperature 102.5 F and blood glucose 180 mg/dL were enrolled. Foals were administered anti-TNF antibody ACTH levels were measured at Day 0 (baseline) and over time (variable by storage method). Plasma samples were stored in either -80 C, -20 C or samples placed between ice packs and stored at -20 C. Plasma samples were stored at -80 C for 3, 7, 30, 60 and 90 days, or stored at -20 C for 3, 7, 30 and 60 days, or stored between ice packs at -20 C for 3 and 7 days prior to determination of resting ACTH concentration. Plasma samples were shipped to the Animal Health Diagnostic Center, Cornell University, Ithaca, NY for measurement of resting ACTH concentration. Within each storage method, ACTH levels over time were compared to baseline (nonfrozen Day 0 plasma) using a paired t-test (i.e. each horse serving as its own control and p < 0.05 was considered statistically significant).