Selected article for: "depth read and exome sequencing"
Title: 2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018
  • Document date: 2018_10_25
    • ID: 60ceejq1_264
    Snippet: Genomic DNA was extracted from the tumor tissues of three dogs that were histologically diagnosed with HS. For the analysis of germline DNA sequences, genomic DNA was also extracted from normal tissues or peripheral blood of these dogs. Whole exome sequencing was performed using Illumina NextSeq 500. The alignment of processed reads to the canine reference genome (CanFam 3.1) was carried out using Genome Analysis Toolkit. VarScan was used for cal.....
    Document: Genomic DNA was extracted from the tumor tissues of three dogs that were histologically diagnosed with HS. For the analysis of germline DNA sequences, genomic DNA was also extracted from normal tissues or peripheral blood of these dogs. Whole exome sequencing was performed using Illumina NextSeq 500. The alignment of processed reads to the canine reference genome (CanFam 3.1) was carried out using Genome Analysis Toolkit. VarScan was used for calling of the somatic variants in each dog and SnpEff was used for the annotations of the variants. The extracted somatic variants were filtered by the read depth and the putative impact for the gene functions. The variants in the genes known to be associated with the pathogenesis of human tumors were validated using Sanger sequencing.

    Search related documents:
    Co phrase search for related documents
    • DNA sequence and exome sequencing: 1
    • DNA sequence and gene function: 1, 2, 3, 4, 5
    • DNA sequence and genomic dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • exome sequencing and Genome Analysis Toolkit: 1
    • exome sequencing and genomic dna: 1, 2, 3, 4, 5, 6, 7
    • gene function and genomic dna: 1, 2