2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

Based on HE staining alone, 41/62 cats (66.1%) were classified as having IBD and 21/62 (33.9%) as having LSA. After consideration of IHC and clonality, 13/62 cats were classified as having IBD (21.0%) and 49/62 as having LSA (79.0%). Thirteen of 26 cases (50.0%) diagnosed as "IBD" and 15/15 cases (100%) diagnosed as "possible LSA" or "probable LSA" based on HE staining alone were subsequently diagnosed with LSA after the addition of IHC and clonality testing. Using Cohen';s Kappa statistic (k), agreement between USI and LSI samples was moderate (k = 0.65) based on HE staining alone, and also moder- Six healthy adult research beagles with a subclinical Giardia spp. infection and a C. canis co-infection were administered FPP as directed by the manufacturer by mouth, daily, for 3 days. Fecal samples were collected 7 and 3 days before FPP (days -7 and -3), on the first day of administration (day 0), and 4, 14, and 21 days after the start of administration (days 4, 14, 21). Fecal DNA was extracted and qPCR assays were performed to assess the abundance of total bacteria, Faecalibacterium, Turicibacter, E. coli, Streptococcus, Blautia, Fusobacterium, and C. hiranonis and to calculate a previously published dysbiosis index (DI). Bacterial groups and DI were compared across time points using the Friedman test and Dunn';s post-test as appropriate. Statistical significance was set as p < 0.05.