2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

In the control group, 1 known missense SNP was identified in exon 1/10 of the SLC22A5 gene, which has been identified to be associated with IBD using both approaches. This SNP was present in 7/20 con- In the case only group, we found one missense SNP (moderate impact, deleterious based on SIFT score) and one in the splice region (low impact, with 1/28 cases sequenced). Seventeen SNPs (5 known and 12 new ones) were identified within 1kb upstream of gene TSS coordinates and 32 SNPs (7 known and 25 new ones) downstream. Two of these SNPs with modifier impact were located within 1kb upstream of IL-3, a hematopoetic cytokine driving the development of myeloid stem cells, which was previously identified to be associated with IBD in both approaches that we used (enrichment and list of genes involved in human IBD). Two SNPs were found downstream of PDLIM (a protein with cysteine-rich double zinc fingers involved in proteinprotein interaction and cytoskeletal organisation) and IL-13 (involved in IgE synthesis, chitinase up-regulation and hyperresponsiveness of mucosal surfaces) and one new SNP was found downstream of IL-4.