2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

In conclusion, LR is beneficial in preventing transfusion reactions and storage lesion. However, IR of canine whole blood was not suitable for long-term storage because of increasing factors of CXCL-8 and storage lesion. IR of leukoreduced blood is beneficial to avoid immune reaction, however, storage lesion should be considered upon storage. Results of these studies identify that a majority of normal and diseased dogs harbor a rich and diverse bile microbiome. This microbiota is likely to contain previously unrecognized beneficial as well as deleterious bacteria. Tailoring bile culture media to routinely identify these bacteria is likely to improve our understanding and diagnosis of canine hepatobiliary disease. The objective of this study was to determine whether increases in these potential biomarkers were associated with moderate to severe fibrosis or necroinflammatory activity in dogs with various hepatopathies. Forty-four client-owned dogs with clinical evidence of liver disease and 10 healthy purpose-bred dogs were enrolled, all undergoing liver biopsies by laparoscopy or laparotomy. Serum IL-6, CCL2, CRP, AST and ALT were measured within one week before scheduled liver biopsy; liver histopathology was evaluated using the METAVIR scoring system used in human medicine, blinded to clinical presentation. Median serum IL-6 was approximately twice as high in dogs with high fibrosis scores (15.5 pg/ml; range, 1.4 to 235 pg/mL) compared to dogs with low fibrosis scores (7.6 pg/mL; range, 1.4 to 148.1 pg/mL), with marginal significance (P = 0.05).