2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

PH was a negative prognostic factor in dogs with mitral valve regurgitation. Our results suggest that MVP is an effective treatment for dogs with PH secondary to mitral valve disease. Further study is needed to clarify long-term prognosis and treatment of patients that exhibit residual PH after surgery. Detection of equine kidney injury in initial stages, when intervention is likely to be most successful, is hindered by limited markers of early renal damage in horses. N-acetyl-β-D-glucosaminidase (NAG), a lysosomal enzyme in renal tubular cells that is released into urine during tubular insult, has shown promise for early identification of acute kidney injury in humans and other species. The aims of this study were to validate an assay for urinary NAG (uNAG) in horses and to correlate uNAG index with other markers of renal tubular dysfunction. An enzymatic assay was validated using coefficients of variation (CV), spike and recovery, and linearity of dilution. Urine and plasma were collected from 7 nonazotemic and 7 azotemic horses. Spearman rank correlation and Wilcoxon rank sum tests were used to compare uNAG index with plasma creatinine, urinary fractional excretion of sodium (FE Na ), and urinary gamma-glutamyltransferase (uGGT) index.