Author: Pandya, Gagan A.; Holmes, Michael H.; Sunkara, Sirisha; Sparks, Andrew; Bai, Yun; Verratti, Kathleen; Saeed, Kelly; Venepally, Pratap; Jarrahi, Behnam; Fleischmann, Robert D.; Peterson, Scott N.
Title: A bioinformatic filter for improved base-call accuracy and polymorphism detection using the Affymetrix GeneChip® whole-genome resequencing platform Document date: 2007_11_15
ID: 16tii0ha_29
Snippet: A schematic representation of the chip design used to represent the F. tularensis genome is depicted in Figure 5 . The F. tularensis LVS genome sequence defined our reference and was represented on chips A-E and the majority of chip F. Unique sequences present in strain SCHU S4, together with two plasmid sequences, were added to the remainder of chip F. Our chip design, based on sequence information from two strains, enables coverage of a large n.....
Document: A schematic representation of the chip design used to represent the F. tularensis genome is depicted in Figure 5 . The F. tularensis LVS genome sequence defined our reference and was represented on chips A-E and the majority of chip F. Unique sequences present in strain SCHU S4, together with two plasmid sequences, were added to the remainder of chip F. Our chip design, based on sequence information from two strains, enables coverage of a large number of strains. Approximately 91% of the F. tularensis double-stranded unique genome can be resequenced with this design from strains belonging to holarctica (type B) and tularensis (type A) subtypes.
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