Author: Banu, Nasirah; Chia, Adeline; Ho, Zi Zong; Garcia, Alfonso Tan; Paravasivam, Komathi; Grotenbreg, Gijsbert M.; Bertoletti, Antonio; Gehring, Adam J.
Title: Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections Document date: 2014_2_25
ID: 44w6omdp_22
Snippet: In addition to optimizing TCR expression we also focused on augmenting redirected T cell function. Instead of using cytokines or specific T cell populations during transduction 30,31 , we added TLR agonists to the culture to mimic pathogen signals encountered during infection. Addition of the TLR ligands had only a minor effect on TCR expression. We observed a slight increase in pentamer positive CD8 T cells with ssRNA40 but the most significant .....
Document: In addition to optimizing TCR expression we also focused on augmenting redirected T cell function. Instead of using cytokines or specific T cell populations during transduction 30,31 , we added TLR agonists to the culture to mimic pathogen signals encountered during infection. Addition of the TLR ligands had only a minor effect on TCR expression. We observed a slight increase in pentamer positive CD8 T cells with ssRNA40 but the most significant difference was observed in the function of the redirected T cell population. We observed increases in the frequency of IFN-c producing cells with polyI:C (TLR-3) and ssRNA40 . This increase in IFN-c1 cells was related to increased polarization.
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