Selected article for: "bovine serum and relative humidity"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_10
    Snippet: For standard experiments, the Human Embryonic Kidney 293 (HEK-293, ATCC CRL 1573) and Chinese Hamster Ovary K1 (CHO-K1, ATCC CCL-61) were routinely grown at 37 • C in 5% CO 2 atmosphere at 100% relative humidity. Cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4 mM L-alanyl-Lglutamine, 10% fetal bovine serum (FBS), 1% non-essential amino-acids, 1% sodium pyruvate, 1% penicillin and streptomycin and 0.25% fun.....
    Document: For standard experiments, the Human Embryonic Kidney 293 (HEK-293, ATCC CRL 1573) and Chinese Hamster Ovary K1 (CHO-K1, ATCC CCL-61) were routinely grown at 37 • C in 5% CO 2 atmosphere at 100% relative humidity. Cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4 mM L-alanyl-Lglutamine, 10% fetal bovine serum (FBS), 1% non-essential amino-acids, 1% sodium pyruvate, 1% penicillin and streptomycin and 0.25% fungizone.

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