Selected article for: "gel loading buffer and NTPase assay"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_21
    Snippet: NTPase assay. NP868R recombinant protein (0.5 M) was incubated with 0.5 Ci [␣-32 P]-GTP or [␣-32 P]-ATP in 50 mM Tris-HCl (pH 8.5), 5 mM DTT, 5 mM KCl supplemented or not with 2.5 mM MgCl 2 and incubated at 37 • C. Reaction was stopped by adding an equal volume of formamide/EDTA gel-loading buffer, and hydrolysis products were separated over a 20% polyacrylamide/8 M urea gel prior to phosphorimaging using FLA3000 instrument (Fuji, Tokyo, Ja.....
    Document: NTPase assay. NP868R recombinant protein (0.5 M) was incubated with 0.5 Ci [␣-32 P]-GTP or [␣-32 P]-ATP in 50 mM Tris-HCl (pH 8.5), 5 mM DTT, 5 mM KCl supplemented or not with 2.5 mM MgCl 2 and incubated at 37 • C. Reaction was stopped by adding an equal volume of formamide/EDTA gel-loading buffer, and hydrolysis products were separated over a 20% polyacrylamide/8 M urea gel prior to phosphorimaging using FLA3000 instrument (Fuji, Tokyo, Japan).

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