Selected article for: "anti rabbit and polyclonal antibody"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_26
    Snippet: Membranes with transferred proteins were blocked with 5% skim milk powder in PBS, then incubated with the rabbit polyclonal F7425 anti-FLAG primary antibody (1:1000; Sigma-Aldrich, Saint-Louis, MO, USA), then with antirabbit IgG-conjugated horseradish peroxidase NA9340V antibody (1:10000; GE Healthcare). Bands were visualized using the SuperSignal West Pico Chemiluminescent Substrate solution (Thermo Scientific) and scanned with the Fusion XPRESS.....
    Document: Membranes with transferred proteins were blocked with 5% skim milk powder in PBS, then incubated with the rabbit polyclonal F7425 anti-FLAG primary antibody (1:1000; Sigma-Aldrich, Saint-Louis, MO, USA), then with antirabbit IgG-conjugated horseradish peroxidase NA9340V antibody (1:10000; GE Healthcare). Bands were visualized using the SuperSignal West Pico Chemiluminescent Substrate solution (Thermo Scientific) and scanned with the Fusion XPRESS gel imager (Vilber Lourmat, Marne-la-Vallée, France). Molecular weights were determined using the Novex Sharp Pre-stained Protein Standard color markers (Thermo Fisher).

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