Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method Document date: 2018_3_23
ID: 4nphwznx_10
Snippet: After 48 to 72 h of transfection, the cell culture supernatant was collected, placed into a 50 mL centrifuge tube, and centrifuged at 4 o C and 5,000 × g for 10 min. The supernatant was then collected, filtered through a 0.45 μm filter, and transferred into a new 50 mL centrifuge tube. Finally, the filtrate was transferred into centrifugal filter devices and centrifuged at 4 o C and 5,000 × g for 10 min. The liquid in the bottom layer was disc.....
Document: After 48 to 72 h of transfection, the cell culture supernatant was collected, placed into a 50 mL centrifuge tube, and centrifuged at 4 o C and 5,000 × g for 10 min. The supernatant was then collected, filtered through a 0.45 μm filter, and transferred into a new 50 mL centrifuge tube. Finally, the filtrate was transferred into centrifugal filter devices and centrifuged at 4 o C and 5,000 × g for 10 min. The liquid in the bottom layer was discarded, and the filtrate further centrifuged at 4 o C and 5,000 × g for 30 min. The solution in the top layer of the filter device was the virus concentrate. The viruses were aliquoted and stored at −80 o C.
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