Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species

Escandon, Paulina; Heatley, J Jill; Berghman, Luc R; Tizard, Ian; Musser, Jeffrey MB

For Mallard samples, the mean dot signal intensity obtained using positive Mallard plasma was significantly different (P<0.05) between all the secondary antibodies; the anti-duck IgY secondary antibody generated the greatest signal intensity value of 18,098, while the anti-macaw IgY secondary antibody generated the least signal intensity value of 561. The signal intensity of the positive plasma dot was significantly greater (P<0.05) than that of the negative plasma dot when using anti-bird, anti-chicken, and anti-duck IgY secondary antibodies. The signal intensity value did not differ significantly between the positive and negative samples, 561 and 297, respectively, when using anti-macaw IgY secondary antibodies.