Title: Compartmentation of the Golgi complex: brefeldin-A distinguishes trans- Golgi cisternae from the trans-Golgi network Document date: 1990_9_1
ID: 47k2yobm_13
Snippet: CHO clone 1021 cells were metabolically labeled for 60 min in the presence of 10 ttg/rnl BFA and then chased for 4 h in the presence or absence of 100 ~tg/ml cycloheximide, shown previously to block protein synthesis by 98 % (34) . Samples were then analyzed as described in Fig. I and above......
Document: CHO clone 1021 cells were metabolically labeled for 60 min in the presence of 10 ttg/rnl BFA and then chased for 4 h in the presence or absence of 100 ~tg/ml cycloheximide, shown previously to block protein synthesis by 98 % (34) . Samples were then analyzed as described in Fig. I and above.
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