Selected article for: "absence presence and BFA absence presence"
Title: Compartmentation of the Golgi complex: brefeldin-A distinguishes trans- Golgi cisternae from the trans-Golgi network
  • Document date: 1990_9_1
    • ID: 47k2yobm_22
    Snippet: galactose cannot be added to N-linked oligosaccharides because the oligosaccharides lack penultimate GlcNAc. O-linked sugar assembly is unaffected, since the O-linked sugar acceptor would be GalNAc, rather than GlcNAc, As shown in Fig. 2 , man6P receptors isolated from CHO clone 15B cells, incubated in the presence or absence of BFA for 3 h, did not bind to RCA-I columns. Failure of these receptors to bind to RCA-I columns was not due to the pres.....
    Document: galactose cannot be added to N-linked oligosaccharides because the oligosaccharides lack penultimate GlcNAc. O-linked sugar assembly is unaffected, since the O-linked sugar acceptor would be GalNAc, rather than GlcNAc, As shown in Fig. 2 , man6P receptors isolated from CHO clone 15B cells, incubated in the presence or absence of BFA for 3 h, did not bind to RCA-I columns. Failure of these receptors to bind to RCA-I columns was not due to the presence of sialic acid-blocked, O-linked gaiactose, because neuraminidase treatment did not increase the RCA-I binding capacity of the isolated receptors (not shown). We conclude that man6P receptors within the ER acquire N-linked galactose in BFA-treated CHO cells. This is consistent with recent results of Lippincott-Schwartz et al. (29) who used indirect immunofluorescence to show the essentially quantitative redistribution of galactosyltransferase to the ER in BFAtreated cells.

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